Skip to main content

Service Categories

 

 

BIOINFORMATICS

ROSALIND - Fast Genomics Data Analysis - OnRamp

Service:

rosalind

ROSALIND – Video how it works

Capability:

The ROSALIND™ platform provides a biologist-friendly experience with instant results to visualize, interpret and discover more in your data. ROSALIND™ offers researchers, biologists and life scientists’ solutions to analyze their own data without needing prior bioinformatics experience. ROSALIND is intended to speed up and simplify the process of genomic data analysis from experiment setup and QC to interactive data visualization and interpretation.

> Create your free account here!

Assays:

  • NanoString assays
  • RNA-seq
  • ChiP-seq
  • Small RNA-seq

Pricing:

  • Inside* user rate: between $30 - $50 per unit (first time analysis training included)
  • Outside user rate: custom quote

*Inside users include UC San Diego, Salk Institute for Biological Studies, Sanford Burnham Prebys Medical Discovery Institute, and La Jolla Institute for Immunology.Core Facility: Stem Genomics Core Human Embryonic Stem Cell Core

Core Facility: Stem Genomics Core

ELECTROPHYSIOLOGY

Meastro MEA

Instrument: 

20190802_120339.jpg

 

Capability:

Measuring the extracellular field potential (EFP) of hiPSC-derived cardiomyocytes can be used to detect drug-induced effects on the electrophysiology of the cells. EFPs can be obtained noninvasively using microelectrode array (MEA) technology without the need for dyes. The multi-well Maestro™ MEA system (Axion BioSystems) enables EFP recordings in various multi-well plates.

 

Core Facility: Human Embryonic Stem Cell Core

ELECTROPORATION

Amaxa Nucleofector

Instrument: 

amaxa

Amaxa Nucleofector

Schedule Type: 

Equipment

Capability:

The Nucleofector Device is an apparatus which delivers unique electrical parameters. The electrical parameters are different from any other commercially available electroporation device and are especially developed to facilitate DNA transport into the cell nucleus. Each electrical setting is displayed as a distinct program which has been adapted to the requirements of a particular cell type.

 

Core Facility: Human Embryonic Stem Cell Core

FLOW CYTOMETRY

BDInflux Sorter

Instrument: 

influx

BD Influx

Schedule Type: 

Flow Cytometry

Capability:

The BD Influx fluidics system features a unique acoustical coupling in the nozzle assembly to reliably create droplets for sorting, while ensuring low shear stress to optimize cell viability, even at high pressures. To support aseptic sorting, the fluidics path is easily removed and replaced with the optional disposable fluidics kit.

Core Facility: Human Embryonic Stem Cell Core

Aria Fusion Sorter

Instrument:

Aria-Fusion-Sorter.jpg

Aria Fusion Sorter

The fusion of safety, performance, and sorting

Capability:

The BD FACSAria™ Fusion flow cytometer delivers exceptional multicolor performance and ease-of-use. First introduced as the BD FACSAria™ sorter in 2003, this sorting knowhow is combined with best-in-class biosafety expertise for a comprehensive advanced cell sorter and biosafety solution. The fully integrated biosafety cabinet meets emerging operator and sample protection requirements as well as global standards for bioprotection.

Core Facility: Human Embryonic Stem Cell Core

BD LSR Fortessa

Instrument: 

fortessa

BD LSRFortessa

Schedule Type: 

Flow Cytometry

Capability:

The BD LSRFortessa can be ordered with up to four lasers—blue, red, violet, and UV—which provides a flexibility in laser wavelengths so assay design can be optimized using the latest fluorescent dyes and substrates. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today’s assay requirement.

Core Facility: Human Embryonic Stem Cell Core

BD LSRFortessa X-20

Instrument: 

fortessa

BD LSRFortessa™ X-20

Designed for limited space and boundless potential

The BD LSRFortessa™ X-20 cell analyzer delivers high-performance multicolor analysis with the most compact footprint in its class at just 30" x 29" (76.2 x 73.7 cm) and a height of 30" (76.2 cm).

The analyzer can be configured with up to 5 lasers to detect up to 20 parameters simultaneously to support ever increasing demands in multicolor flow cytometry. A wide range of up to 34 laser choices is available as excitation sources, including blue, red, violet, yellow-green, and UV

Contact us today to learn more about trading in your BD TM LSR II system and take advantage of our trade-in programs.

The BD LSRFortessa™ X-20 fits most flow cytometry analyzer needs, and can be ordered with up to 5 lasers-most commonly, blue, red, violet, yellow-green, and UV-which provides flexibility in laser wavelengths. This allows researchers to optimize assay design by selecting the latest fluorescent dyes and antibodies in experimental protocols. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet today's assay requirements.

The BD LSRFortessa X-20 is designed to support your current assay requirements. In addition, as future needs arise, optical capability can be added or upgraded. 

A compact footprint and height of 76.2 x 73.7 x 76.2 cm (30 x 29 x 30 in.) allows the BD LSRFortessa X-20 to fit easily on the benchtop for the cost-effective use of valuable laboratory space. In addition to the reduced size, design enhancements provide easier access to bandpass filters and mirrors, simplifying changes in the instrument configuration.To improve experimental workflow, the optional BD TM High Throughput Sampler (HTS) provides rapid, fully automated sample acquisition from microtiter plates. In high-throughput mode, the HTS option can speed through a 96-well plate in less than 15 minutes with less than 0.5% sample carryover from one well to the next. Low carryover is essential to ensure data quality in research applications.

 

Core Facility: Human Embryonic Stem Cell Core

BD ARIA ii Sorter

INSTRUMENT:

BD-ARIA-ii-Sorter.jpeg

Meet the new BD FACSAria II high-speed, fixed-alignment cuvette cell sorter.

Capability:

The first generation BD FACSAria™ system brought the complex world of cell sorting to a broader audience of researchers. Now the new BD FACSAria II system makes cell sorting even easier and more accessible.

Designed with input from customers around the world, the new BD FACSAria II improves ease of use, flexibility, and aseptic capability to make it simply the best choice for consistent results in sorting across a broad range of applications.

The BD FACSAria II incorporates the unique fixed alignment technology that revolutionized cell sorting over five years ago with the introduction of the BD FACSAria. Fixed alignment minimizes startup time and improves reproducibility. Gel coupling to the objective lens improves collection efficiency and increases sensitivity and resolution needed for multicolor sorting applications.

Core Facility: Human Embryonic Stem Cell Core

GENE EXPRESSION

nCounter® MAX Analysis System

Instrument:

Fully automated and easy-to-use, the nCounter® MAX Analysis System provides everything you need to cost-effectively complete your projects in record time. More accurate and highly multiplexed than qPCR and simpler than NGS, the nCounter® MAX allows for digital examination of multiple pathways in a single tube in an extraction-free workflow requiring only 15 minutes of hands on time. Accelerate your research by spending less time on sample prep and perform your own data analysis using the included nSolver Analysis Software.

  • Boost Productivity: Intuitive workflow with only fifteen minutes of hands-on time from sample to data. Separate Digital Analyzer and Prep Station units help eliminate bottlenecks in sample processing and data collection.
  • Simplify Analysis: No need for a specialized Bioinformaticist. Results generated as direct counts and reported in a standard CSV file that can be imported into your favorite application or use the included nSolver Analysis Software for convenient data analysis.
  • Detect Small Fold Changes: Eliminate cDNA synthesis, amplification, and library prep so you experience less technical variation in your assay and reduce the need for experimental replicates.
  • Expandable Throughput: Add a second Prep Station when needed and double your capacity to match a growing number of users. This configuration enables processing of up to 96 lanes per day and 384 samples with sample plexing.
  • Secure Access: An enterprise software package is included for laboratories that require enhanced security. Control user access, automate data flow, and generate audit logs.
  • Unattended Data Collection: The Digital Analyzer can load up to six cartridges at a time for unattended scanning overnight. Random access enables users to pause data collection to load or remove additional cartridges without waiting for the run to finish.

*Inside users include UC San Diego, Salk Institute for Biological Studies, Sanford Burnham Prebys Medical Discovery Institute, and La Jolla Institute for Immunology.

Resources:

 

Note: Some consumables are also available for purchase. Please inquire with Dr. Elsa Molina for more information.

 

Core Facility: Stem Genomics Core

Biomark HD - Fluidigm

MOLECULAR IMAGING INSTRUMENTS

11.7 T horizontal Bruker BioSpec MRI

Instrument


11.7 T horizontal Bruker Biospec MRI.

Capabilities
  • 16 cm room temperature bore
  • Gradients with strength 750 mT/m, 90 mm clear-bore access
  • Laser guided motorized positioning system
  • Beds and stereotaxic hardware
  • Ambient temperature control and body temperature monitoring
  • Monitoring system for ECG and respiration triggering acquisitions
  • Multiple Bruker RF coils:
    • Circularly polarized 1H volume coil with inner diameter 38 mm (T12247V3)
    • Circularly polarized 1H volume coil with inner diameter 72 mm (T11232V3)
    • Body volume coil (T11440V3)
    • Surface array coil (T11766V3)
    • Surface array coil (T11473V3)
    • Double-tuned 1H/19F volume coil (T12800V3)
    • Double-tuned 1H/19F volume coil (RAPID Biomedical)
    • Double-tuned 1H/19F surface coil (T11620V3)
    • Double-tuned 1H/31P surface coil (T11619V3)
    • Double-tuned 1H/13C surface coil (T11618V3)

 

Core Facility: MICS

Bioluminescense/flourescence Perkin Elmer IVIS Spectrum System with 3D imaging and spectral unmixing

Instrument


Perkin Elmer IVIS Spectrum system

CapabilitiesThe IVIS® Spectrum in vivo imaging system combines 2D optical and 3D optical tomography in one platform. The system uses leading optical technology for preclinical imaging research and development ideal for non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals.

An optimized set of high efficiency filters and spectral un-mixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. It also offers single-view 3D tomography for both fluorescent and bioluminescent reporters that can be analyzed in an anatomical context using our Digital Mouse Atlas or registered with our multimodality module to other tomographic technologies such as MR, CT or PET.

For advanced fluorescence pre-clinical imaging, the IVIS Spectrum has the capability to use either trans-illumination (from the bottom) or epi-illumination (from the top) to illuminate in vivo fluorescent sources. 3D diffuse fluorescence tomography can be performed to determine source localization and concentration using the combination of structured light and trans illumination fluorescent images. The instrument is equipped with 10 narrow band excitation filters (30nm bandwidth) and 18 narrow band emission filters (20nm bandwidth) that assist in significantly reducing autofluorescence by the spectral scanning of filters and the use of spectral unmixing algorithms. In addition, the spectral unmixing tools allow the researcher to separate signals from multiple fluorescent reporters within the same animal.

Features & Benefits

  • High-sensitivity in vivo imaging of fluorescence and bioluminescence
  • High throughput (5 mice) with 23 cm field of view
  • High resolution (to 20 microns) with 3.9 cm field of view
  • Twenty eight high efficiency filters spanning 430 – 850 nm
  • Supports spectral unmixing applications
  • Ideal for distinguishing multiple bioluminescent and fluorescent reporters
  • Optical switch in the fluorescence illumination path allows reflection-mode or transmission-mode illumination
  • 3D diffuse tomographic reconstruction for both fluorescence and bioluminescence
  • Ability import and automatically co-register CT or MRI images yielding a functional and anatomical context for your scientific data.
  • NIST traceable absolute calibrations
  • Gas anesthesia inlet and outlet ports
  • Class I Laser Product

 

Core Facility: MICS

PET-SPECT-CT Integrated Trimodal Siemens Inveon Preclinical System

Instrument

 

Capabilities

  Visit here for more information on the Inveon PET-SPECT-CT Siemens trimodal system. 

Core Facility: MICS

Ultrasound Visualsonics Vevo 2100

Instrument


Vevo 2100

The Vevo® 2100 system expands the functionality, flexibility and image quality of the earlier Vevo 770 system

Capabilities

The Vevo 2100 operates at frequencies never before achieved with solid-state array transducers. 

The new MicroScan™ transducers provide increased frame rates, superb contrast, unrivaled resolution and a wider field of view.

Features Include: 

  • Superior resolution and image uniformity through entire field of view
  • 30 micron resolution
  • Frame rates up to 740 fps

 

Expandable Imaging and Processing Options

  • Color and Power Doppler Modes for blood flow quantification 
  • M-Mode single line acquisition for high-temporal resolution in LV functional analysis
  • 3D-Mode Imaging & Volume Analysis
  • Nonlinear Contrast Imaging
  • VevoStrain™ Analysis software for cardiac research
  • Advanced measurements & quantification

Software analysis and data management:

Key Features

  • Ideal for long term studies with ability to add images/data to an existing study
  • View images simultaneously for consistency and comparison 
  • Reserve time on the system - perform data analysis on an off-line computer

Data Management and Export

Analyze, label, annotate and archive images and studies through a flexible, open-architecture system. 

  • Export a list of acquired studies/series/images to paste into lab book 
  • Easily export with and without annotations/measurements (without duplication of data) 
  • Easily export with and without physiological parameters (without duplication of data) 

Export data as TIFF, BMP, AVI uncompressed AVI, MS Video9 and DICOM

 

Core Facility: MICS

MICROSCOPY

Confocal Laser Scanning Biological Microscope FV1000 FluoView with Two-photon – Olympus

Instrument: 

olympus

Olympus FV1000 Confocal

Schedule Type: 

Microscopes

Capability:

This model features the world's first twin scan system. This system enables time-resolved spectroscopy of living intercellular contents and also makes it possible to analyze objects down to a 2nm wavelength resolution.

 

Core Facility: Stem Genomics Core

Confocal Microscope LSM 780 – Zeiss

Instrument:

zeiss lsm 710

Zeiss LSM 780 Confocal Microscope

Capability:

The PTC laser concept of LSM 780 is revolutionary in confocal microscopy: the system uses pigtailed lasers directly on the scanning head. With up to eight ports, you couple near-UV, VIS and IR lasers in many combinations.

The 34-channel QUASAR detector by Carl Zeiss allows the optimal acquisition strategy for various emission spectra, without being bound to filters and dichroic mirrors – you simply configure your experiment in the software.

The spectral signal you obtain is an excellent basis for high-resolution spectral imaging and the parallel detection of up to ten colors. If needed, you can direct any parts of the spectrum to the required detector.

When it comes to excitation, the new TwinGate main beam splitter has two dichroic filter wheels and high transmission of up to 100 combinations of laser lines – allowing for the optimum fluorescence excitation of your samples.

The spectral recycling loop of LSM 780 amplifies signals by feeding the unseparated part of the emission light through the grid a second time before adding it to the complete signal. When used with the QUASAR detector, you will reduce dark noise by two-thirds and significantly improve the sensitivity of your microscope.

 

Core Facility: Stem Genomics Core

Leica Confocal SPE

Instrument:

Image result for leica spe

Leica Confocal SPE

Capability:

High-resolution Spectral Confocal for Daily Research and Routine Examinations Leica TCS SPE

The Leica TCS SPE confocal is a true point-scanning, spectral system at an affordable price for fluorescence imaging of live or fixed cells.

Providing all of the features needed for routine confocal techniques,it offers excellent quality imaging. The system is easy to use, and first results are quickly achieved, even by confocal novices.  The common Leica LAS X interface facilitates direct operation.

With its small footprint, the Leica TCS fits in every laboratory at common room conditions. The Leica TCS SPE is the only confocal in its class that provides true spectral detection suitable for lambda scan.

Core Facility: Stem Genomics Core

Incucyte S3

Equipment

Incucyte S3

Capability

Innovation Never Takes a Break

Analyze your cells for days, weeks or even months as they sit stationary in the stable environment of your tissue culture incubator.

IncuCyte fast-tracks scientific discovery by combining lab-tested protocols and reagents with powerful, automated image acquisition and analysis. With IncuCyte’s user-friendly interface and robust instrument portfolio, any cell biologist can gain dynamic insights into the health, morphology, movement and function of their cell models.

 

Core Facility: Stem Genomics Core

SAMPLE QC

TapeStation 2200 - Agilent

Qubit 3.0 Fluorometer - Thermo Fisher Scientific

Instrument:

qubit

Qubit 3.0 Fluorometer

Capability: 

Powerful, dual-core processor quickly and accurately quantifies DNA, RNA, and protein, in <5 seconds per sample. Uses as little as 1 μL of sample.

 

Core Facility: Stem Genomics Core

NanoDrop 2000 Spectrophotometer - Thermo Fisher Scientific

Li-Cor Odyssey

Instrument

Odyssey CLx

Li-Cor Odyssey

Get clearer answers with accurate, reproducible Western blots and many other assays.

Capability: 

Measure concentration and purity of DNA, RNA or protein samples.

Do more with consistent, reproducible digital imaging technology that has propelled the Odyssey imager family to over 10,000 peer-reviewed publications. See the benefits of stable NIR fluorescence and detect multiple targets in the same lane. Get the most accurate results with over 6 logs of linear dynamic range, so you can make more discoveries.

Core Facility: Human Embryonic Stem Cell Core

SAMPLE SHEARING & ISOLATION

Covaris E220 Focused Ultra Sonication - Covaris

SEQUENCING

MiSeq – illumina

Instrument:

miseq illumina

Illumina - MiSeq

Capability: 

Highly multiplexed amplicon sequencing, Targeted Resequencing, Small Genome Sequencing, De Novo sequencing, CHiP Seq, Small RNA Sequencing, Library QC for HiSeq

*MiSeq not recommended for deep sequencing or sequencing of large genomes. For deep sequencing on the HiSeq, please contact IGM

 

Core Facility: Stem Genomics Core

SINGLE-CELL LIBRARY PREPARATION

10x Chromium Controller – 10x Genomics

Instrument:

slide-0 

10x Chromium Controller 
Capability:

Compact, sleek, efficient.

The compact, sleek Chromium Controller rapidly and efficiently combines large partition numbers with a massively diverse barcode library to generate >100,000 barcode-containing partitions in a matter of minutes. The Chromium Controller fits on a standard laboratory bench and allows a user to run any Chromium Solution, from genome to single cell analysis.

Download Technology Brochure 

 

Core Facility: Stem Genomics Core

Rhapsody Single-Cell Analysis System – BD

Instrument:

Rhapsody Single-Cell Analysis System
Capability:

Analyze the expression of hundreds of genes across tens of thousands of single cells in parallel

The BD Rhapsody Single-Cell Analysis System profiles the gene expression of thousands of single cells, with predesigned or customized assays flexible enough to meet any experimental need in an efficient system that reduces experimentation time and sequencing costs.

 

Core Facility: Stem Genomics Core 

C1 Single Cell Auto Prep – Fluidigm

THERMOCYCLER

ProFlex 3x32-well PCR System - Thermo Fisher Scientific

Instrument

ProFlexâ„¢ 3 x 32-well PCR System

ProFlex 3x32-well PCR System

Capabilities:

The ProFlex™ 3x32-Well PCR System is the latest in our line of Applied Biosystems® thermal cyclers. The ProFlex™ system combines the reliability and performance you’ve come to expect from Applied Biosystems® instruments with the flexible configuration and control features that adapt to your research needs.

Request a demo.

The ProFlex™ 3x32-Well PCR System allows you to: 

• Run three experiments at once with the 3x32-well, 0.2-mL block
• Interchange the 3x32-well block with another block type if your throughput needs change
• Access the system remotely (and conveniently) through a mobile app
• Program the instrument in seconds with a simple-to-use touchscreen interface
• Simulate your old instrument with Thermal Simulation modes
• Optimize your PCR quickly and efficiently with better-than-gradient VeriFlex™ blocks

Core Facility: Stem Genomics Core

Thermal Cycler C1000 Touch 96w - Bio-Rad

Instrument

Core Equipment

Thermal Cycler C1000 Touch 96w

Capabilities:

The C1000 Touch thermal cycler offers superior performance and a large color touch screen for easy programming. This fully modular platform is able to accommodate different throughput needs with easily interchangeable reaction modules that swap in seconds without tools. Each PCR module has a fully adjustable heated lid that supports a wide range of vessels and sealers, including low-profile and standard-height PCR plates.

Features and Benefits

  • Quick and easy protocol programming — 2 programming options, including the Protocol Autowriter, which automatically generates customized standard, fast, and ultrafast protocols
  • Easy protocol editing — the large color touch screen and redesigned navigation make editing and running protocols easy
  • Flexible platform — change throughput capability by linking up to 3 additional S1000 thermal cyclers or adding PC control for up to 32 cyclers
  • Advanced file management — USB flash drive compatibility allows universal protocol transfer and unlimited data storage
  • Thermal gradient — quickly and easily identify optimal annealing temperatures by using the programmable temperature gradient

Applications and Uses

  • Amplification/PCR
  • Cloning
  • Cycle sequencing
  • Gene expression studies
  • Mutagenesis

The C1000 Touch thermal cycler can be used with six interchangeable reaction modules, including:

  • A gradient-enabled 96-well fast module
  • A gradient-enabled 96–deep well module
  • A gradient-enabled dual 48/48-well fast module that allows 2 independently controlled protocols to be run side by side in a single bay
  • A gradient-enabled 384-well module for high throughput
  • 2 modules for real-time PCR detection

 

Core Facility: Stem Genomics Core

BioRad CFX 384

Instrument

Core Equipment

Thermal Cycler C1000 Touch 96w

Capabilities:

The CFX384 Touch Real-Time PCR Detection System is a powerful and precise real-time PCR instrument in a 384-well format, for researchers who require both ease of use and rapid, high-throughput data acquisition. The CFX384 Touch System utilizes accurate temperature control and sensitive, 4-color detection optics to deliver reliable and repeatable qPCR results for singleplex or multiplex reactions.

Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to four-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful software, the CFX384 Touch System was designed to advance your qPCR.

Key Features and Benefits

With the CFX384 Touch Real-Time PCR Detection System you can:

  • Obtain precise quantification and multiplex target discrimination – optical system uses long-lasting solid-state technology with 5 filtered LEDs and 5 filtered photodiodes
  • Configure the system to fit your laboratory needs – optimized design lets you run without a computer, run up to 4 systems from 1 computer, or integrate with the CFX Automation System II for higher throughput
  • Confirm system performance and data reliability – internal system qualification and system test software, automated data quality control, and various validation tool services are available
  • Obtain accurate results with volumes as low as 3 µl – minimize sample and reagent usage
  • Easily integrate with your laboratory information management system (LIMS) – integrate with any LIMS using built-in LIMS file management
  • Combine the CFX384 Touch System with good laboratory practice standards – use CFX Maestro Software, Security Edition, which complies with U.S. FDA 21 CFR Part 11 regulations, for data collection and analysis

 

Core Facility: Human Embryonic Stem Cell Core

BioRad CFX 96

Instrument

Core Equipment

BioRad CFX 96

Capabilities:

The CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions.

Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use the included CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to five-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful yet easy-to-use software, the CFX96 Touch System is designed to advance your qPCR.

Key Features and Benefits

With the CFX96 Touch Real-Time PCR Detection System you can:

  • Set up your system quickly — easy installation and factory-calibrated optics
  • Minimize sample and reagent use — up to 5-target multiplexing with sample volumes as low as 10 µl
  • Optimize reactions in a single run — thermal gradient feature
  • Analyze data faster — visualize all run data at once and export only the data you need in the format you want
  • Use advanced data analysis tools — normalized gene expression using CFX Maestro Software
  • Configure the system to fit your needs — run without a computer, run up to 4 systems from 1 computer, or integrate with the CFX Automation System II for higher throughput
  • Combine the CFX96 Touch System with good laboratory practice standards — use CFX Maestro Software, Security Edition for data collection and analysis to simplify compliance with U.S. FDA 21 CFR Part 11 regulations

Flexible CFX System Configurations Meet Your Throughput Needs

The CFX Automation System II works with all CFX Real-Time PCR Detection Systems to enable walk-away, high-throughput qPCR operation. Integrate this robotic plate handler with one or two CFX Systems to maximize throughput while maintaining a compact footprint.

 

Core Facility: Human Embryonic Stem Cell Core

ULTRACENTRIFUGE

Optima L-80 XP Ultracentrifuge 1

Instrument: 

optima

Optima L-80 XP Ultracentrifuge 1

 

Capability:

The ultracentrifuge is a centrifuge optimized for spinning a rotor at very high speeds. The L-80 XP is capable of generating 602,000 x g at speeds of up to 80,000 rpm. The L-80 XP, along with a broad range of rotors, tubes, and supplies shortens run times for a wide range of applications. The L-80 XP’s special design eliminates the need for chlorofluorocarbons or other ozone-depleting coolants for drive and chamber cooling.

 

Instrument

Core Equipment

Thermal Cycler C1000 Touch 96w

Capabilities:

The C1000 Touch thermal cycler offers superior performance and a large color touch screen for easy programming. This fully modular platform is able to accommodate different throughput needs with easily interchangeable reaction modules that swap in seconds without tools. Each PCR module has a fully adjustable heated lid that supports a wide range of vessels and sealers, including low-profile and standard-height PCR plates.

Features and Benefits

  • Quick and easy protocol programming — 2 programming options, including the Protocol Autowriter, which automatically generates customized standard, fast, and ultrafast protocols
  • Easy protocol editing — the large color touch screen and redesigned navigation make editing and running protocols easy
  • Flexible platform — change throughput capability by linking up to 3 additional S1000 thermal cyclers or adding PC control for up to 32 cyclers
  • Advanced file management — USB flash drive compatibility allows universal protocol transfer and unlimited data storage
  • Thermal gradient — quickly and easily identify optimal annealing temperatures by using the programmable temperature gradient

Applications and Uses

  • Amplification/PCR
  • Cloning
  • Cycle sequencing
  • Gene expression studies
  • Mutagenesis

The C1000 Touch thermal cycler can be used with six interchangeable reaction modules, including:

  • A gradient-enabled 96-well fast module
  • A gradient-enabled 96–deep well module
  • A gradient-enabled dual 48/48-well fast module that allows 2 independently controlled protocols to be run side by side in a single bay
  • A gradient-enabled 384-well module for high throughput
  • 2 modules for real-time PCR detection

 

Core Facility: Human Embryonic Stem Cell Core

 

Optima L-80 XP Ultracentrifuge 2

Instrument: 

optima 2

Optima L-80 XP Ultracentrifuge 2

 

 

Capability:

The ultracentrifuge is a centrifuge optimized for spinning a rotor at very high speeds. The L-80 XP is capable of generating 602,000 x g at speeds of up to 80,000 rpm. The L-80 XP, along with a broad range of rotors, tubes, and supplies shortens run times for a wide range of applications. The L-80 XP’s special design eliminates the need for chlorofluorocarbons or other ozone-depleting coolants for drive and chamber cooling.

 

Core Facility: Human Embryonic Stem Cell Core