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FLOW: Instruments and Services

Amaxa Nucleofector

Instrument: 

amaxa

Amaxa Nucleofector

Schedule Type: 

Equipment

Capability:

The Nucleofector Device is an apparatus which delivers unique electrical parameters. The electrical parameters are different from any other commercially available electroporation device and are especially developed to facilitate DNA transport into the cell nucleus. Each electrical setting is displayed as a distinct program which has been adapted to the requirements of a particular cell type.

Pricing:

$10

Avanti J-e Centrifuge

Instrument: 

avanti

Avanti J-e Centrifuge

Schedule Type: 

Equipment

Capability:

The Avanti J-E high-performance centrifuge is a refrigerated centrifuge that generates centrifugal forces for processing sample preparations, pelleting, extractions, purifications, concentrations, separation of blood cells and gradients, and more. It features a brushless switched-reluctance drive motor, temperature control system, and selectable acceleration and deceleration rates.

Pricing:

$10

Aria Fusion Sorter

Instrument:

Aria-Fusion-Sorter.jpg

Aria Fusion Sorter

The fusion of safety, performance, and sorting

Capability:

The BD FACSAria™ Fusion flow cytometer delivers exceptional multicolor performance and ease-of-use. First introduced as the BD FACSAria™ sorter in 2003, this sorting knowhow is combined with best-in-class biosafety expertise for a comprehensive advanced cell sorter and biosafety solution. The fully integrated biosafety cabinet meets emerging operator and sample protection requirements as well as global standards for bioprotection.

Pricing:

$85

Core Facility: Human Embryonic Stem Cell Core

BD FACSAria II

Instrument: 

aria ii

BD FACSAria II

Schedule Type: 

Flow Cytometry

Capability:

The FACSAria II Sorter is a digital sorter enabling high speed sorting and multicolor analysis. The instrument accommodates four air-cooled lasers and is capable of multicolor sorting of up to eleven parameters. It has digital acquisition rates of up to 70,000 events/second.

Pricing:

$85

BD FACSCanto 2 HTS

Instrument: 

canto

BD FACSCanto

Schedule Type: 

Flow Cytometry

Capability:

The FACSCanto Flow Cytometer combines a pattented optical design and manufacturer upgrades for simultaneous data acquisition of eight fluorescent signals, two scatter parameters, digital electronics for processing up to 10,000 events per second, and a novel sample injection system supporting carry over of less than 0.1%.  The Hi Throughput System (HTS) can acquire 96 well and 384 well plaste acquistion with the Canto 2.

Pricing:

$45

BD Influx

Instrument: 

influx

BD Influx

Schedule Type: 

Flow Cytometry

Capability:

The BD Influx fluidics system features a unique acoustical coupling in the nozzle assembly to reliably create droplets for sorting, while ensuring low shear stress to optimize cell viability, even at high pressures. To support aseptic sorting, the fluidics path is easily removed and replaced with the optional disposable fluidics kit.

Pricing:

$85

BD LSRFortessa™ X-20

Instrument: 

fortessa

BD LSRFortessa™ X-20

Designed for limited space and boundless potential

The BD LSRFortessa™ X-20 cell analyzer delivers high-performance multicolor analysis with the most compact footprint in its class at just 30" x 29" (76.2 x 73.7 cm) and a height of 30" (76.2 cm).

The analyzer can be configured with up to 5 lasers to detect up to 20 parameters simultaneously to support ever increasing demands in multicolor flow cytometry. A wide range of up to 34 laser choices is available as excitation sources, including blue, red, violet, yellow-green, and UV

Contact us today to learn more about trading in your BD TM LSR II system and take advantage of our trade-in programs.

The BD LSRFortessa™ X-20 fits most flow cytometry analyzer needs, and can be ordered with up to 5 lasers-most commonly, blue, red, violet, yellow-green, and UV-which provides flexibility in laser wavelengths. This allows researchers to optimize assay design by selecting the latest fluorescent dyes and antibodies in experimental protocols. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet today's assay requirements.

The BD LSRFortessa X-20 is designed to support your current assay requirements. In addition, as future needs arise, optical capability can be added or upgraded. 

A compact footprint and height of 76.2 x 73.7 x 76.2 cm (30 x 29 x 30 in.) allows the BD LSRFortessa X-20 to fit easily on the benchtop for the cost-effective use of valuable laboratory space. In addition to the reduced size, design enhancements provide easier access to bandpass filters and mirrors, simplifying changes in the instrument configuration.To improve experimental workflow, the optional BD TM High Throughput Sampler (HTS) provides rapid, fully automated sample acquisition from microtiter plates. In high-throughput mode, the HTS option can speed through a 96-well plate in less than 15 minutes with less than 0.5% sample carryover from one well to the next. Low carryover is essential to ensure data quality in research applications.

 

Pricing:

$45

Core Facility: Human Embryonic Stem Cell Core

BD LSRFortessa

Instrument: 

fortessa

BD LSRFortessa

Schedule Type: 

Flow Cytometry

Capability:

The BD LSRFortessa is equipped with four lasers: blue, red, violet, and yellow—green . This 4 - laser system provides flexibility for optimizing assays using multiple fluorescent dyes and substrates. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today’s assay requirement.

Pricing:

$45

FlowJo software on hESCCF Computer

Instrument: 

FlowJo software on hESCCF Computer

Schedule Type: 

Equipment

Capability:

N/A

Pricing:

$0

Odyssey CLx

Instrument: 

Odyssey CLx

Odyssey CLx

Schedule Type: 

Equipment

Capability:

The Odyssey CLx System can be used for many applications, including Western blot analysisEMSAprotein arraysIn-Cell AssaysOn-Cell Westernsin vivo imagingCoomassie gel documentationDNA gel documentation, and tissue section analysis.

Pricing:

$15

Olympus FV1000 Confocal

Instrument: 

olympus

Olympus FV1000 Confocal

Schedule Type: 

Microscopes

Capability:

This model features the world's first twin scan system. This system enables time-resolved spectroscopy of living intercellular contents and also makes it possible to analyze objects down to a 2nm wavelength resolution.

Pricing:

$40

Optima L-80xP Ultracentrifuge

Instrument: 

Optima L-80Xp

Optima L-80 XP Ultracentrifuge 1

Schedule Type: 

Equipment

Capability:

The ultracentrifuge is a centrifuge optimized for spinning a rotor at very high speeds. The L-80 XP is capable of generating 602,000 x g at speeds of up to 80,000 rpm. The L-80 XP, along with a broad range of rotors, tubes, and supplies shortens run times for a wide range of applications. The L-80 XP’s special design eliminates the need for chlorofluorocarbons or other ozone-depleting coolants for drive and chamber cooling.

Pricing:

$10

Optima L-80 XP Ultracentrifuge 2

Instrument: 

optima 2

Optima L-80 XP Ultracentrifuge 2

Schedule Type: 

Equipment

Capability:

The ultracentrifuge is a centrifuge optimized for spinning a rotor at very high speeds. The L-80 XP is capable of generating 602,000 x g at speeds of up to 80,000 rpm. The L-80 XP, along with a broad range of rotors, tubes, and supplies shortens run times for a wide range of applications. The L-80 XP’s special design eliminates the need for chlorofluorocarbons or other ozone-depleting coolants for drive and chamber cooling.

Pricing:

$10

Other

CELLS

  • Mycoplasma test (Up to 3 samples at one time)
  • Mycoplasma test (Up to 7 samples at one time)

OTHER

  • 3.5mm dish sleeve
  • normocin aliquot
  • 0.1% Gelatin 250mls

Perkin Elmer Envision

Instrument: 

perkin

Perkin Elmer Envision

Schedule Type: 

Equipment

Capability:

The 2104 EnVision Multilabel Reader is our state-of-the-art dual-detector model and among the fastest HTS readers in the market. The instrument includes two detectors enabling simultaneous dual wavelength reading, below emission reading, barcode readers, a high speed light source and adjustment of measurement height function.

Pricing:

$20

Plastics

Please contact Jesus Olvera.

The UC San Diego hESCCF charges users the price provided by the vendor, plus tax and shipping. The following is a list of our current plastics.

SPECIAL PLASTICS

  • 6 well ultra-low binding plate
  • 96 well v-shaped spin EB dish
  • Stem Cell Roller
  • AggreWell
  • 8 well slide
  • Nucleocassette
  • Cell Counter Slide
  • Lab-Tek Chamber Slide w/Cover Permanox Slide Sterile

TC Hoods

Instrument: 

tchood

TC Hoods

Schedule Type: 

TC Hoods

Capability:

N/A

Pricing:

$15

VivaView

Instrument: 

vivaview

VivaView

Schedule Type: 

Microscopes

Capability:

The VivaView FL provides an integrated and motorized inverted microscope to allow long-term time-lapse imaging in a constant and optimized environment. Multiple locations in up to 8 samples can be imaged simultaneously with fluorescence or Differential Interference Contrast. Simple, intuitive computer operation removes all the difficulty previously associated with configuring a live cell imaging system.

Pricing:

$10

Zeiss AxioImager

Instrument: 

zeiss

Zeiss AxioImager

Schedule Type: 

Microscopes

Capability:

This item is a research microscope that features excellent optics and homogeneous illumination in both transmitted light and fluorescence applications. It is a smart control concept for ergonomic working conditions and multi-user operation – with the option of control via optional TFT technology. The AxioImager allows encoding: read-out magnification, illumination and contrast settings, and transfer to ZEN Imaging software.

Pricing:

$10

Pricing

Equipment

instrument picture

Product Name

Cost Per Hour

Amaxa Nucleofector

$10

Avanti J-e Centrifuge   

$10

FlowJo software on HESCCF Computer

$0

Li-Cor Odyssey 

$15

Optima L-80 XP Ultracentrifuge 1   

$10

Optima L-80 XP Ultracentrifuge 2   

$10

Perkin Elmer Envision

$20

Real Time PCR 

$20

Flow Cytometry  

instrument picture

Product Name

Cost Per Hour

BD FACS Aria II                  

$85

BD FACS Aria Fusion

$85

BD FACS Canto 2 HTS 

$45

BD Influx

$85

BDLSR Fortessa

$45

BDLSR Fortessa X-20 HTS

$45 

Microscopes

instrument picture

Product Name

Cost Per Hour

CellInsight                     

$30

Olympus FV1000 Confocal

$40

VivaView

$10

Zeiss AxioImager

$10

TC Hoods

instrument picture

Product Name

Cost Per Hour

TC Hoods

$15

Training Courses

instrument picture

Product Name

Cost Per Hour

FloJo 1 on 1 Training

$50

Introduction to Confocal Microscopy

$50