FLOW: Instruments and Services

Instrument: 

Amaxa Nucleofector

Schedule Type: 

Equipment

Please Contact Cody Fine and Vu Nguyen for machine location after signing up.

Capability:

The Nucleofector Device is an apparatus which delivers unique electrical parameters. The electrical parameters are different from any other commercially available electroporation device and are especially developed to facilitate DNA transport into the cell nucleus. Each electrical setting is displayed as a distinct program which has been adapted to the requirements of a particular cell type.

Pricing:

$10

Instrument: 

Avanti J-e Centrifuge

Schedule Type: 

Equipment

Please Contact Cody Fine and Vu Nguyen for machine location after signing up.

Capability:

The Avanti J-E high-performance centrifuge is a refrigerated centrifuge that generates centrifugal forces for processing sample preparations, pelleting, extractions, purifications, concentrations, separation of blood cells and gradients, and more. It features a brushless switched-reluctance drive motor, temperature control system, and selectable acceleration and deceleration rates.

Pricing:

$10

Instrument:

Aria Fusion Sorter

The fusion of safety, performance, and sorting

Capability:

The BD FACSAria™ Fusion flow cytometer delivers exceptional multicolor performance and ease-of-use. First introduced as the BD FACSAria™ sorter in 2003, this sorting knowhow is combined with best-in-class biosafety expertise for a comprehensive advanced cell sorter and biosafety solution. The fully integrated biosafety cabinet meets emerging operator and sample protection requirements as well as global standards for bioprotection.

Pricing:

$85

Core Facility: Human Embryonic Stem Cell Core

Instrument: 

BD FACSAria II

Schedule Type: 

Flow Cytometry

Please Contact Cody Fine and Vu Nguyen for machine location after signing up.

Capability:

The FACSAria II Sorter is a digital sorter enabling high speed sorting and multicolor analysis. The instrument accommodates four air-cooled lasers and is capable of multicolor sorting of up to eleven parameters. It has digital acquisition rates of up to 70,000 events/second.

Pricing:

$85

Instrument: 

BD FACSCanto

Schedule Type: 

Flow Cytometry

Please Contact Cody Fine and Vu Nguyen for machine location after signing up.

Capability:

The FACSCanto Flow Cytometer combines a pattented optical design and manufacturer upgrades for simultaneous data acquisition of eight fluorescent signals, two scatter parameters, digital electronics for processing up to 10,000 events per second, and a novel sample injection system supporting carry over of less than 0.1%.  The Hi Throughput System (HTS) can acquire 96 well and 384 well plaste acquistion with the Canto 2.

Pricing:

$45

Instrument: 

BD Influx

Schedule Type: 

Flow Cytometry

Please Contact Cody Fine and Vu Nguyen for machine location after signing up.

Capability:

The BD Influx fluidics system features a unique acoustical coupling in the nozzle assembly to reliably create droplets for sorting, while ensuring low shear stress to optimize cell viability, even at high pressures. To support aseptic sorting, the fluidics path is easily removed and replaced with the optional disposable fluidics kit.

Pricing:

$85

Instrument: 

BD LSRFortessa™ X-20

Designed for limited space and boundless potential

The BD LSRFortessa™ X-20 cell analyzer delivers high-performance multicolor analysis with the most compact footprint in its class at just 30" x 29" (76.2 x 73.7 cm) and a height of 30" (76.2 cm).

The analyzer can be configured with up to 5 lasers to detect up to 20 parameters simultaneously to support ever increasing demands in multicolor flow cytometry. A wide range of up to 34 laser choices is available as excitation sources, including blue, red, violet, yellow-green, and UV

Contact us today to learn more about trading in your BD ^TM LSR II system and take advantage of our trade-in programs.

The BD LSRFortessa™ X-20 fits most flow cytometry analyzer needs, and can be ordered with up to 5 lasers-most commonly, blue, red, violet, yellow-green, and UV-which provides flexibility in laser wavelengths. This allows researchers to optimize assay design by selecting the latest fluorescent dyes and antibodies in experimental protocols. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet today's assay requirements.

The BD LSRFortessa X-20 is designed to support your current assay requirements. In addition, as future needs arise, optical capability can be added or upgraded. 

A compact footprint and height of 76.2 x 73.7 x 76.2 cm (30 x 29 x 30 in.) allows the BD LSRFortessa X-20 to fit easily on the benchtop for the cost-effective use of valuable laboratory space. In addition to the reduced size, design enhancements provide easier access to bandpass filters and mirrors, simplifying changes in the instrument configuration.To improve experimental workflow, the optional BD ^TM High Throughput Sampler (HTS) provides rapid, fully automated sample acquisition from microtiter plates. In high-throughput mode, the HTS option can speed through a 96-well plate in less than 15 minutes with less than 0.5% sample carryover from one well to the next. Low carryover is essential to ensure data quality in research applications.

Pricing:

$45

Core Facility: Human Embryonic Stem Cell Core

Instrument: 

BD LSRFortessa

Schedule Type: 

Flow Cytometry

Please Contact Cody Fine and Vu Nguyen for machine location after signing up.

Capability:

The BD LSRFortessa is equipped with four lasers: blue, red, violet, and yellow—green . This 4 - laser system provides flexibility for optimizing assays using multiple fluorescent dyes and substrates. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today’s assay requirement.

Pricing:

$45

Instrument: 

FlowJo software on hESCCF Computer

Schedule Type: 

Equipment

Please Contact Cody Fine and Vu Nguyen for machine location after signing up.

Capability:

N/A

Pricing:

$0

Equipment

Capability

Innovation Never Takes a Break

Analyze your cells for days, weeks or even months as they sit stationary in the stable environment of your tissue culture incubator.

IncuCyte fast-tracks scientific discovery by combining lab-tested protocols and reagents with powerful, automated image acquisition and analysis. With IncuCyte’s user-friendly interface and robust instrument portfolio, any cell biologist can gain dynamic insights into the health, morphology, movement and function of their cell models.

Relevant Links

Spheroid/Organoid Prep:

Click Here

Technical Documents:

https://www.essenbioscience.com/en/resources/documents/

Publication lists:

https://www.essenbioscience.com/en/resources/publications/

Webinar Database:

https://www.essenbioscience.com/en/resources/webinars/

Core Facility: Human Embryonic Stem Cell Core

Instrument: 

Odyssey CLx

Schedule Type: 

Equipment

Please Contact Cody Fine and Vu Nguyen for machine location after signing up.

Capability:

The Odyssey CLx System can be used for many applications, including Western blot analysis, EMSA, protein arrays, In-Cell Assays, On-Cell Westerns, in vivo imaging, Coomassie gel documentation, DNA gel documentation, and tissue section analysis.

Pricing:

$15

Instrument: 

Optima L-80 XP Ultracentrifuge 1

Schedule Type: 

Equipment

Please Contact Cody Fine and Vu Nguyen for machine location after signing up.

Capability:

The ultracentrifuge is a centrifuge optimized for spinning a rotor at very high speeds. The L-80 XP is capable of generating 602,000 x g at speeds of up to 80,000 rpm. The L-80 XP, along with a broad range of rotors, tubes, and supplies shortens run times for a wide range of applications. The L-80 XP’s special design eliminates the need for chlorofluorocarbons or other ozone-depleting coolants for drive and chamber cooling.

Pricing:

$10

Instrument: 

Optima L-80 XP Ultracentrifuge 2

Schedule Type: 

Equipment

Please Contact Cody Fine and Vu Nguyen for machine location after signing up.

Capability:

The ultracentrifuge is a centrifuge optimized for spinning a rotor at very high speeds. The L-80 XP is capable of generating 602,000 x g at speeds of up to 80,000 rpm. The L-80 XP, along with a broad range of rotors, tubes, and supplies shortens run times for a wide range of applications. The L-80 XP’s special design eliminates the need for chlorofluorocarbons or other ozone-depleting coolants for drive and chamber cooling.

Pricing:

$10

CELLS

• Mycoplasma test (1 to 3 samples - $42)
• Mycoplasma test (7 samples - $52.50)

OTHER

• 3.5mm dish sleeve
• normocin aliquot
• 0.1% Gelatin 250mls

The UC San Diego hESCCF charges users the price provided by the vendor, plus tax and shipping. The following is a list of our current plastics.

SPECIAL PLASTICS

• 6 well ultra-low binding plate
• 96 well v-shaped spin EB dish
• Stem Cell Roller
• AggreWell
• Cell Counter Slide

Instrument: 

TC Hoods

Schedule Type: 

TC Hoods

Please Contact Cody Fine and Vu Nguyen for machine location after signing up.

Capability:

N/A

Pricing:

$15

Instrument: 

Tecan Infinite m200 Pro

Schedule Type: 

Equipment

Please Contact Cody Fine and Vu Nguyen for machine location after signing up.

Capability:

"The Tecan Infinite m200 Pro is an easy-to-use luminometer with the capacity for absorbance, luminescence, luminescence dual colors, and fluorescent intensity measurements. The instrument features a drag-and-drop software that allows for convenient configuration of any experiments, including the option for incubation, movement of scan plate, and adjustment of measurement of height function."

Pricing:

Free

Instrument: 

VivaView

Schedule Type: 

Microscopes

Please Contact Cody Fine and Vu Nguyen for machine location after signing up.

Capability:

The VivaView FL provides an integrated and motorized inverted microscope to allow long-term time-lapse imaging in a constant and optimized environment. Multiple locations in up to 8 samples can be imaged simultaneously with fluorescence or Differential Interference Contrast. Simple, intuitive computer operation removes all the difficulty previously associated with configuring a live cell imaging system.

Pricing:

$10

Instrument: 

Zeiss AxioImager

Schedule Type: 

Microscopes

Please Contact Cody Fine and Vu Nguyen for machine location after signing up.

Capability:

This item is a research microscope that features excellent optics and homogeneous illumination in both transmitted light and fluorescence applications. It is a smart control concept for ergonomic working conditions and multi-user operation – with the option of control via optional TFT technology. The AxioImager allows encoding: read-out magnification, illumination and contrast settings, and transfer to ZEN Imaging software.

Pricing:

$10