Stem Cell Genomics and Microscopy Core: Instruments and Services

Zeiss LSM 780 Confocal Microscope

Capability:

The new illumination and detection design of the LSM 780 confocal microscope brings complete freedom to your fluorescence microscopy. You work with up to ten dyes and use continuous spectral detection across the complete wavelength range. LSM 780 enables confocal microscopy for a wide variety of applications.With the inverse Axio Observer from Carl Zeiss, LSM 780 offers you unrivalled confocal microscopy in cell and developmental biology. Upright stands such as Axio Imager or Axio Examiner offer you have all the equipment you need to record neurobiological, physiological and developmental relationships to an exceptional standard.

Objectives:

10x, 20x, 40x, 60x, and 100x

Pricing:

• Self service (inside* user rate): $30 per hour
• Self service (outside user rate): $60 per hour

*Inside users include UC San Diego, Salk Institute for Biological Studies, Sanford Burnham Prebys Medical Discovery Institute, and La Jolla Institute for Immunology.

Please inquire with Dr. Elsa Molina for more information.

Confocal Laser Scanning Biological Microscope FV1000 FluoView with Two-photon - Olympus

Capability: 

The Olympus FluoView FV1000 opens new frontiers in live cell imaging with advanced technologies that provide improved sensitivity and high scan speeds for imaging live organisms with minimal specimen damage.

The innovative, Olympus-developed SIM Scanner incorporates two independent, fully synchronized laser scanners in a single compact design for simultaneous laser stimulation and confocal observation. Rapid cellular reactions that occur during or immediately following laser stimulation may now be captured without a time lag. Such studies of rapid cellular responses can provide important insight into how various cellular mechanisms operate. This makes the FV1000 ideal for such advanced techniques as FRAP, FLIP, uncaging, photoactivation and photoconversion.

Pricing:

• Self service (inside* user rate): $45 per hour
• Self service (outside user rate): $90 per hour

*Inside users include UC San Diego, Salk Institute for Biological Studies, Sanford Burnham Prebys Medical Discovery Institute, and La Jolla Institute for Immunology.

Please inquire with Dr. Elsa Molina for more information.

Keyence BZ-X800 Fluorescence Microscope

VIdeo overview of the system

Capability: 

Fluorescence observation can be performed anywhere in the lab just by closing the front panel to create a built-in darkroom. Exposure time can be individually adjusted while overlaying images in real time on one screen. A fully-motorized setup allows the user to control the XY/Z-axis stage, aperture stop, phase contrast rings, neutral density filter, monochrome/color camera switching, shutter speed, filter turret, and lens turret. The large, fully-motorized XY stage allows for rapid scanning and observation of slides, dishes, and multi-well plates. This allows researchers to observe their samples faster and more efficiently, leading to quicker results. The Navigation and Stage View functions facilitate the simultaneous observation of multiple samples, without ever losing sight of areas of interest. The cooled monochrome CCD enables highly-sensitive imaging of weak fluorescent signals, which allows for accurate brightness quantification for applications measuring protein expression. Additionally, a color filter allows one-click transition between monochrome and high-resolution color observation. Unlike a color camera, the color filter is not attached to the CCD, which can lead to distortions during fluorescence viewing. Quickly capture a wide-area image of an entire tissue section just by specifying its outer edges. All fields-of-view will be automatically captured and merged together to create a seamless, high-resolution image of up to 50,000 x 50,000 pixels. XY stitching can be combined with the Z-stack function to create fully focused images of thick or unevenly cut sections.  Overview of the BZ-X800/BZ-X8101-5BZ-X800 User’s ManualGetting Started1Multi-dimensional time lapsePerform time-lapse experiments with bright field, phase contrast, and fluorescence samples by specifying the capture settings and interval time. By using the Multi-point function, up to 99 different areas of interest can be captured in a given interval, each with unique capture settings such as magnification, exposure time, etc. This drastically improves the efficiency of time-lapse experiments, allowing for multiple samples to be observed simultaneously throughout the duration of the experiment. Capture and analyze multiple samples simultaneously and collectively by the image cytometer function. Easy and direct captures can be made with the same condition to multiple wells.Analysis conditions can be specified easily by referring to images. Since many samples can be analyzed simultaneously, more reliable statistical data can be acquired effectively. The chronological change of a living cell can be confirmed by combination with the time lapse.

Pricing:

• Self service : $30 per hour

Please inquire with Dr. Elsa Molina for more information.